An exceptional oddity occurs in Africa, Australia, and South America during dry seasons: an oval-shaped, cocoon-like structure that is found under the ground which when gently broken open produces a long and curled live fish like something out of a sci-fi movie! One can continue to dig the surrounding area and find hundreds more of these fish peacefully resting under the ground.

This fish, bestowed with life in a cocoon of mud it fashions under the ground, is called the “lungfish.” It has been discovered that lungfish can survive thanks to their wonderful features for several years in cocoon-like pits under extremely severe and arid conditions. The secrets to this amazing phenomenon lie in the fish’s exclusive respiratory system.

While lungs are the respiratory organs for vertebrates that live on land, the respiratory organs known as gills have been granted to most aquatic animals. Lungfish actually breathe through both gills and lungs. This is why they are also known as dipnoid, a special type of “double breathing” species which have one or two lungs next to their gills. These are not actually lungs but are instead air sacs surrounded by capillaries. They function as lungs when the fish need to utilize them. In particular, these sacs are activated when the water in the fish’s environment dries up. Lungfish are able to overcome drought periods that may last many years by remaining buried in mud and breathing through these God-gifted air sacs.

There are six lungfish species known to be living in Africa, South America, and Australia. The fish resembles the eel due to their prominently long and cylindrical body structure. Studies have shown that the African species tend to be the largest and can reach up to two meters in length, while its Australian and South American counterparts can grow up to 1.25 meters. Fossil prints indicate that African lungfish emerged roughly 400 million years ago and are thus often called “living fossils.”

Just as everything is created and equipped in accordance with their exact needs, so is the lungfish. Lungfish do not have dorsal fins. Their chest and abdominal fins have features that make it easier for the fish to crawl on the ground. The fins are long, adhesive, and highly mobile, and their tips have a very delicate sense of touch. Moreover, the fish’s olfactory and taste receptor cells, and its lateral lines that detect pressure and turbulence, have been created to be extremely sensitive. These structures function as senses that support the fish’s weak sense of vision. Additionally, there are electro-receptors on the fish’s nose that help it to easily perceive their surroundings and aid with their survival.

As the water recedes during the dry season, each lungfish first digs a tunnel for itself in the slime and settles in it. At the top of the tunnel is a porous cover that allows air to smoothly enter and exit. It is within this tunnel that the fish fashions a cocoon for itself made out of a mucous it spews which is designed to keep moisture and allows air in. After these meticulous preparations, the fish goes into a summer sleep that is similar to hibernation. Its physiology also changes during this period as its metabolic speed is lowered to 1/60 of the normal circumstances and its body functions are brought almost entirely to a halt. The fish spends this whole period in this way until the next rainy season arrives.

The lungfish, no doubt, also need to maintain their energy during this deep sleep. They can generate energy by dissolving a portion of their own muscle tissues as a sort of “fat reserve.” Consumption of muscle tissue as food leads them to lose about 3 centimeters in size during one season, and they also lose almost half of their weight during extensive droughts.

An incredible respiratory mechanism

Animals of the underwater world use their gills for breathing. The multi-functional gills are delicately structured to allow the dissolved oxygen in the water to be released into the bloodstream and have the carbon dioxide removed. The gills are also instrumental in several processes including gas exchange, osmoregulation, acid-base adjustment, and nitrogenous waste disposal. A fish normally only has oxygenated blood in its gills. In the fish classified as dipnoid, the extremely thin membrane covering the inner surface of the air sac connected to the circulation system is conducive for gas exchange, thus it helps respiration by acting like a lung.

Different from other species, the lungfish are equipped with a highly developed exclusive respiratory mechanism that enables them to live both in water and on land. While some fish species that can breathe air do so by using basic gas sacs, the system in lungfish is far more complex.

There is a main canal in their breathing organs that serve as a type of de facto lungs, and this canal is surrounded with numerous chambers that increase when expanded but decrease in size. Most chambers have a central space linked with a ventilation duct. The inner surfaces of these structures are lined with numerous honeycomb-like air sacs fed by fine capillaries. A gas exchange occurs in these small vesicles, thus maximizing the surface area on which this gas exchange takes place. Additionally, these fish also have a circulation dedicated to the air sac which functions as a separate lung that is the same as in land vertebrates.

Lungfish go up the surface to breathe and position their heads in a way so that the tip of their nose touches the water’s surface. Meanwhile, they open their mouths and draw air from just above the water. During this process, they usually make a characteristic sound that varies slightly across species. The lungfish in Australia are a little different from the others; they breathe air through their nasal openings while their mouths are closed. In addition, the lungfish in Australia breathe air in shorter periods and they use their lungs only in high activation periods in their natural environment. This is why their gills are very strong since they have only one lung. Due to intense use under severe climatic conditions, the African and South American lungfish have two lungs instead of one and their gills are much smaller than that of other lungfish.

With unique features bestowed to their respiratory system, lung and gill structures so they can survive under extreme conditions, lungfish are a treasure for us to think and marvel on the mysteries found in nature.

References

• Olga Carvalho, Carlos Gonçalves. “Comparative Physiology of the Respiratory System in the Animal Kingdom.” The Open Biology Journal, 2011, 4, 35–46.
• en.wikipedia.org/wiki/Lungfish
• www.britannica.com/animal/lungfish
• www.nationalgeographic.org/media/west-african-lungfish

Truffles are potato-shaped underground mushrooms that grow in all kinds of different environments ranging from high-rise forests of pine, oak, linden, fir and wild hazelnut to scrubs, under bushes, and in steppes and deserts. They maintain a symbiotic relationship by attaching to the roots of certain herbaceous plants and have a unique aroma with a sharp odor. Some cultures nickname truffles as bingos or buckthorns. There is also the belief that the mushrooms sprout faster under lightning or thunder thus earning them the nickname “daughters of thunder.”

Symbiotic association between truffles and plants

Nature thrives in a harmony that is based upon assistance and solidarity. Truffles have a special place in this harmony. For example, as plant seeds germinate and begin to grow into roots, the hyphae of truffles wrap around the plant roots, just like a glove does around fingers, and help the emergence of a special structure called “mycorrhiza” which facilitates food exchange between truffles and plants. Approximately 90% of plants coexist with different types of mushrooms, and some plants even need truffles to survive. Unable to photosynthesize, truffles need plants for organic nutrients, and plants need truffles, especially for the intake of more water and minerals from barren and arid soil.

It is estimated that the mushroom hyphae can be as long as 1 km in the forest soil is estimated to be more than 1 km. Truffles help to increase the contact surface of the tree roots with the soil hundreds of times while ingesting the water and minerals from remote areas where plant roots cannot reach. In this way, as soon as a drop of rain falls on the soil, it is relayed to the benefit of plants with hyphae that are invisibly thin and kilometers long. If this partnership did not exist between truffles and plants then the giant trees that we see in forests would not be able to grow tall enough and would possibly remain as bushes due to a lack of water and minerals from the soil.

Truffles and wildlife

A similar relationship exists between truffles and animals in the forest. Since they are generally active at night and cannot benefit from sunlight sufficiently, wild animals meet their vitamin D needs especially from truffles, an important food source. Recent studies show that some wild animal species survive solely on truffles. A large number of mice, squirrels, bears, deer, rabbits, hedgehogs, and bird species in the US eat truffles along with some monkeys, kangaroos, and bird species in Australia.

While the toadstools in the open can spread their billions of spores to the environment, the spores of underground mushrooms remain confined in their tissues. It is thanks to animals that can spot and extract the mushrooms that these spores spread from their tissues into the rest of the environment. Wild animals can locate, dig up, and eat underground truffles due to the strong and attractive aroma of the mushrooms. On the other hand, the spores that are expelled from the animals’ digestive systems lead to the production of fresh hyphae to partner with new plants via germination. This allows underground mushrooms to have the opportunity to sustain their generation and expand their natural habitat.

The conservation of animal species that face extinction also depends on the preservation of the variety of truffle species. From this point of view, truffles in the natural habitats are the sustenance of wild animals. Bediuzzaman Said Nursi (d. 1960), a renown Turkish religious scholar, said: “Our share is in vineyards and gardens. God Almighty allocated our sustenance there. These wild fruits are the sustenance for the wild animals. We should not touch their portion.” It is also known that Bediuzzaman discouraged his students who came across plenty of apple and pear trees on the mountains from eating those fruits. This approach also sums up a guiding insight about sustainable forestry and ecosystem.

Nutritional value and medical benefits

Truffles are richer in protein and minerals than other mushrooms. Their nutritional value consists of 53-76% water, 9% protein, 7% carbohydrates, and 8% minerals. Although they have high nutritional value, the most important feature making truffles superior to other mushrooms is their distinctive aromatic compounds. Due to their unique aroma, truffles attract the attention of many gourmets. It is no surprise that truffles have an exceptional place in exclusive cuisines.

Since ancient times, the medical benefits of truffles have been frequently reported. For example, Ibn Sina (Avicenna) is known to have recommended truffles for healing weakness, nausea, pain, and wounds.

Prophet Muhammad, peace be upon him, said, “Truffle is a sustenance like manna. Its sap is also a cure for the eyes.”

The belief in the nutritional value of truffles is also common in the Christian world. Between 827 and 844, Pope Gregory IV had advised the consumption of truffles to gain strength in battles.

Current studies have found that truffles contain several compounds that are essential for human health. A study published in 2016 is an important step for chronicling the fact that the extract obtained from truffle mushrooms proved to be useful in healing eye infections.

Economic value

The number of commercial-value truffles collected from natural habitats constantly decreases worldwide: it has dropped from 2000 tons in 1884 to 100 tons in 1990. Today, it is around 40 tons. The main factors of this decline are the destruction of oak forests, climate change, environmental pollution, global warming, and uncontrolled picking.

Despite the dramatic decrease in the number of truffles collected from natural habitats, they are sold between $250 and $4,000 per kilo depending on the type and quality.

Growing truffles

Under current conditions, truffles have to be grown by special means. People must grow the types of truffles that they wish to consume.

The first idea for the production of truffles came from a French farmer named Joseph Talon. At the beginning of the 19th century, Talon planted fresh seeds in the oak patches where truffles grew naturally. The existing truffle hyphae in the soil infiltrated the roots of new oak saplings and led to an increase in the production of truffles in the natural habitat. In the following years, Talon created new truffle production areas by planting the saplings he had already produced in other patches. Talon’s method is still used today.

In the 1960s, a period when there was a great decrease in the production of truffles in natural habitats, studies were conducted to find new methods. The methods developed by French and Italian scientists in the 1970s led to a massive success in truffle production. Truffle spores were inoculated at the root of oak saplings and plants were grown in greenhouses to develop only the desired truffle mycorrhizal system in tree roots. Having ensured that mycorrhiza had settled in the roots, the first truffle began to be harvested four or five years after planting the oak saplings in open areas. These methods have allowed truffle production in countries such as Australia, the USA, and New Zealand where truffles are not grown naturally.

Commercial truffle types

It is estimated that there are about 10,000 different types of truffles in the world with different sizes, colors, structures, and aromas. These mushrooms, which are now listed in restaurant menus and stocked on the shelves of luxury food suppliers, are only some of the truffles numbered in thousands.

Some of the commercial types are listed below:

Tuber magnatum: Known as the white truffle of Italy, it grows in the Alba region as attached on the roots of oak, hornbeam, pine and poplar trees. It is different from all species by its peculiarly pungent aroma. It is known as the most expensive food in the world because it grows in a very limited area and cannot be grown as a cultivated mushroom.

Tuber melanosporum: Known as the winter black truffle, it grows during winter by attaching to the roots of oak, hazelnut, and pine trees in Italy, France, Spain, and the Balkans. It has a distinctive aroma and has a wider growth area as compared to the white truffle. It is the most cultivated type of truffle in different continents of the world.

Tuber aestivum: Known as the summer black truffle, it grows by attaching to the roots of oak, nut, and pine trees in a wide geography spanning from Portugal to Azerbaijan, Morocco to Poland, and Sweden to Afghanistan. It bears lower economic value because it is widely grown and has an easily extracted culture and a lower-density aroma.

Terfezia claveryi: It grows in the spring across steppes and deserts by attaching onto the roots of herbaceous plant species belonging to the genus Helianthemum. It has a unique aroma and has a huge market in the Arab countries. Its culture has started to be extracted in recent years.

Oregon truffle: It grows in winter as attached on the roots of fir trees in Oregon, Washington, and Vancouver. It has white and black types. It has an important market in the U.S despite not being as valuable as the black and white species found in Europe. No results have been obtained from cultural studies yet.

Harvest of truffle

Picking truffles is like picking apples from a tree with the difference that truffles are collected from the root of the tree. It is difficult to understand whether the truffle underground has ripened or not. If the extracted truffle is not ripe enough then its economic value immediately suffers. That is why special dogs are bred to locate truffles. Sensitive to the truffle aroma, these dogs lead their owners by simply pointing out to the places where ripe truffles are found. These dogs are motivated by rewards and are encouraged with better rewards for finding higher quality, larger, and more ripe truffles.

References

• Alhussaini S.M., Saadabi A.M., Hashim K., Al-Ghanayem A.A. (2016). Efficacy of the Desert Truffle Terfezia claveryi to Cure Trachoma Disease with Special Emphasis on Its Antibacterial Bioactivity, Trends in Medical Research, doi: 10.3923/tmr.2016.28.342016, Volume: 11, Issue: 1, pp. 28–34.
• Bukhari, 5708; Muslim, 2049; Abu Dawood, Tibb, 12; Ibn Majah, Tibb, 8.
• Hall I.R., Brown G., Zambonelli A. (2008). Taming the Truffle: The History, Lore, and Science of the Ultimate Mushroom, Timber Press.
• Sahiner Necmeddin, Son Sahitler, Istanbul: Nesil Yayinlari, 2011, Volume 1, pp. 113.
• Trappe M, Claridge AW (2010). “The Hidden Life of Truffles”. Scientific American. April 2010: 78–84.
• Wedén C. (2008). Tryffel. Infotain &Infobooks Sweden AB, Stockholm.

It was around dawn, and the day was not yet fully light. Rain the night before had left a thick cloud covering the scene. The music of bugs and the chanting of birds filled the air. Against a background of a slight breeze, which provided a smooth and continuous whistle, consecutive, rhythmic thumps could be heard. These thumps were the sounds of a giant’s footsteps as he was walking to work early in the morning.

Meanwhile, my grandpa and his friends were enjoying the water on the walkway. But a hot day was soon going to make the walkway the death bed of many of his friends. Aside from the heat of the day, the footsteps of the giants would bring to an end the lives of many others. Neither he nor his friends were aware of any of this. They were simply enjoying their water on the walkway.

That morning, the giant who was walking to work bent down towards my grandfather, grabbed a thin stick from the ground, and tried to kill him. Not ready for such an attack in the midst of his breakfast, my grandfather was stupefied, did not know what to do. He just spiraled away to escape the giant’s attack, as we always do in case of danger. But all was in vain; the giant would not give up.

Finally, the giant accomplished what he was trying to do, and thus my grandfather was saved from both the heat and the giant footsteps. It turned out that my grandpa had misunderstood the giant. Normally, other giants would walk on us, or would ignore us, and this giant was the same; or so he had thought. However, this one was trying to put the stick under my grandpa to lift him and place him onto the soil where he could be safe. My grandpa said that he appreciated this help more later when he heard his friends dying on that same walkway.

The story of a giant who was unlike others spread from the family to friends, and became known all around. The world underground rejoiced at the existence of this one giant who was not like others… Maybe there were other giants like him, too; and the underground world kept praying for those giants that they might be protected from things they were unaware of, just as that giant had saved my grandfather from things that he was unaware of.

After my grandfather’s times, his story became a nostalgic tale for the elders and a bedtime story for little ones. The world aboveground became worse than the world underground. Instead of long trees above with their roots under ground, you would see huge, tall rocks in both places. The rocks underground provided water to everyone, but the rocks aboveground only provided water to the giants. The roots of the plants and trees used the marrow of the earth to embellish it, but the long channels that the giants sank into the ground were like huge veins draining the earth to death. We ceased praying for them.

One day, we heard screaming among the giants about an asteroid heading directly towards the earth. The only thing they were worried about was the end of their own lives. We were not surprised by this selfish reaction to that event because of their selfish past on the earth. And the asteroid came…

It was foggy again, but not due to clouds; it was quite dark, but not due to dawn. The fog and the darkness were due to dust spread over the face of the earth. We were fortunate to be able to sustain our lives under such harsh conditions. As for the giants, they were not suffering much from the conditions, as they had taken shelter in special chambers built only for them. Being some of the few survivors of the impact, we were of significant use to them. After using the entire earth selfishly, now they were enslaving us. So, they still lacked our prayers for their protection. Could they ever figure out, despite their hardened hearts, why such tragedy had befallen them?

Not long after that time, we witnessed something totally incredible. One of the giants came out of the protection chambers to do something that was completely unexpected. He looked through his goggles at the sky and tried to take a deep breath. As he was coughing because of the dust in the air, he opened the nylon bag he was carrying. He sat on the ground, dug a hole with his hands, and uncovered the thing in the bag: a daisy plant. Was he going to plant it? But why? The chances of its survival were almost none. What benefit would it bring him, anyway, even if it survived for a day? He looked pleased with what he was doing. He even smiled at a worm that was escaping the hole he had dug for the flower. What was going on in his mind?

It was just at the moment he was about to plant the flower that the earth suddenly started shaking like crazy. The planet was dying because of the deep cracks caused by the impact of the asteroid. Now, neither the giant nor the flower had any chance of survival. He did not know what to do for a while; then he started shedding tears: “Oh my God! Oh my God!”

Then he saw the deep cracks forming near him, shaking and growing. It was as if the planet had started shouting at him in thousands of voices. He could not stand the quakes and fell down next to the hole, as if it were he, instead of the flower, that was going to be buried in it. At that moment, this giant was no different from us: lying on the ground, and seeking his way out of this doom. Who was there to help him? His cries were now screams: “Oh my God! Oh my God!”

All of a sudden, he stopped crying and started talking to someone, but we could not see anybody around: “Please, hold me tight. Do not leave me….” He continued, “But I can’t… I can’t….” Then, he tried to reach the flower that had rolled away from him when he fell down. He grabbed it and cradled it at his chest; but struck by a stone falling from one of the nearby rocks, he fell unconscious. As he was sinking into the growing cracks, we uttered a prayer for a giant who had planted a flower in his heart… The last prayer of worms, the last prayer for giants…

Seth Mette has a PhD in Aerospace Engineering and is currently working as a postdoctoral fellow at West Virginia University. He has a special interest in psychological fiction.